Gutegura no Kwiga Antibodies IgY Kurwanya Base Rusange ya Metallases kuva Agkistrodon halys Venom

[Abstract] kandi ikomatanya antigen peptide zabitswe cyane PT1 na PT2, zifitanye isano rya hafi nigikorwa cya enzyme;Hens yakingiwe hamwe na conjugated KLH-PT1 na KLH-PT2 kugirango babone antibodies za IgY.Titer ya IgY n'ibiranga cross-reaction hamwe na Agkistrodon acutus na Agkistrodon ubumara bwa acutus bwakozwe na ELISA na blot yo muburengerazuba;Hanyuma, igikorwa cyo kutabogama cya IgY cyasuzumwe n’ikizamini cyo kurwanya indwara yo kuva amaraso mu mbeba [Ibisubizo] Ibisubizo bya ELISA na blot yo mu Burengerazuba byerekanaga ko anti-KLH-PT1 na anti-Agkistrodon IgY ishobora guhura na Agkistrodon acutus na Agkistrodon acutus ubumara. , kandi ibya nyuma byari bikomeye cyane kuruta ibya mbere;Anti-KLH-PT2 IgY nta reaction igaragara igaragara hamwe nuburozi bwa Agkistrodon acutus nuburozi bwa Agkistrodon muri vitro, ariko ifite uburozi bukomeye butabogama mubushakashatsi bwo kurwanya amaraso muri vivo, kandi buruta cyane ubwa mbere [Umwanzuro] IgY antibody ishobora kwambuka reaction hamwe nuburozi butandukanye bwinzoka zinzoka zateguwe hifashishijwe igishushanyo mbonera rusange cya antigen peptide ya metalloproteinase, yashyizeho urufatiro rwo gutegura uburozi bwihariye, bukora neza, buke buke hamwe na antibodi yuburozi bw’inzoka.