Gutegura no Kwiga Antibodies za IgY kuri Motif Rusange ya Metallase kuva Agkistrodon acutus Venom

Antibody ya IgY irwanya motif isanzwe ya metalloproteinase ikomoka ku bumara bwa Agkistrodon acutus yateguwe kandi ubushakashatsi bwarwo bwagize uruhare mu bikorwa by’ubumara bw’inzoka. , zifitanye isano rya hafi nibikorwa bya enzyme;Inkoko zakingiwe hamwe na KLH-PT1 hamwe na KLH-PT2 zifatanije kugirango zibone antibodiya za IgY.Titer ya IgY nibiranga umusaraba hamwe nuburozi bwa Agkistrodon acutus na Agkistrodon brevius byakozwe mbere na ELISA na blot yo muburengerazuba;Hanyuma, ibikorwa byo kutabogama kwa IgY byasuzumwe n’ikizamini cyo kurwanya indwara yo kuva amaraso mu mbeba [Ibisubizo] ELISA na blot yo mu burengerazuba bwerekanye ko anti KLH-PT1 hamwe n’uburozi bwa Agkistrodon Acutus IgY ishobora kwambuka uburozi bwa Agkistrodon acutus nuburozi bwa Agkistrodon, yari ikomeye cyane kurusha iyambere;Kurwanya KLH-PT2 IgY ntabwo byagize ingaruka zikomeye ku musaraba hamwe na Agkistrodon acutus na Agkistrodon brevius ubumara muri vitro, ariko yerekanye uburozi bukomeye butabogamye mu bushakashatsi bwo kurwanya amaraso muri vivo, kandi bwarutaga cyane ibyambere bibiri [Umwanzuro] Antibody IgY ishobora cross reaction hamwe nuburozi bwinzoka butandukanye buzenguruka inzoka zateguwe mugushushanya peptide ya antigen ihuriweho na metalloproteinase, yashyizeho urufatiro rwo gutegura uburozi bwihariye, bukora neza, buke buke hamwe na spécran yagutse irwanya ubumara bwinzoka ya inzoka.