Gukemura subunits ya trombine nka enzyme Agapurin yo muri Agkistrodon halys uburozi no gukurikirana ibisigazwa bya aside amine

Intego Kumenya N-terminal 15 ibisigisigi bya aside amine ya subunits ebyiri zisa na Agacutin.Ibice bibiri bya molekile ya enzyme bigomba gucikamo ibice kugirango ubone subunit imwe ya peptide ikurikirana kandi ikurikiranye.Uburyo bwogejwe cyane Agacutin yabonetse muburyo bwa biohimiki.Ingero imwe ya subunit yo kumenyekanisha no gukurikiranwa yateguwe na SDS-PAGE itandukanya electrophoreis, subunit adhesive strip kugarura hamwe na tekinoroji ya PVDF membrane electrotransfer.Aba nyuma bagennye ibisigisigi 15 bya aside amine kuri N-terminal ya subunit hakoreshejwe uburyo bwo gutesha agaciro Edman.Ibisubizo Urukurikirane rwa subunit nini (16 kDa) yari DCSSGWSSYEEHQYY, naho iya subunit nto (15 kDa) yari DSSGWSSYEGHEYYV.Umwanzuro Ibisubizo byakurikiranye byerekanaga ko Agacutin yari inzoka nshya yubumara bwinzoka nka enzyme ushakisha ububiko bwa NCBI.