Tumor inhibitory ingaruka yibibyimba byubumara I kuri kanseri yibihaha byabantu A549

Intego: Kwiga ingaruka zokugabanya ibibyimba I (AAVC-I) kubuza ikwirakwizwa ryinshi na apoptose ya kanseri yibihaha byabantu A549.Uburyo: Uburyo bwa MTT bwakoreshejwe kugirango hamenyekane igipimo cya 24h na 48h cyo kubuza selile A549 na AAVC-I muburyo butandukanye.
HE irangi hamwe na Hoechst33258 irangi rya fluorescent ryakoreshejwe mukureba apoptose ituruka kuri morphologie;Immunohistochemie yakoreshejwe mugutahura impinduka mumagambo ya protein ya BAX.Ibisubizo: MTT yerekanye ko AAVC-I ishobora kubuza ikwirakwizwa rya selile A549 mugihe- kandi biterwa na dose.Nyuma yo kuvura AAVCI kuri 24h, kugabanuka kwa nucleus, hyperstaining nucleaux na apoptotic umubiri byagaragaye munsi ya microscopi.Immunohistochemie yerekanaga ko impuzandengo yubucucike bwa optique yiyongereye hamwe no kwiyongera kwibiyobyabwenge, byerekana ko imvugo ya proteine ​​ya BAX yagengwaga nayo.